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DNA Sequence Library for External RNA Controls
DNA Sequence Library for External RNA Controls
Price: $715.00
Unit of Issue: 96 tubes
Unit Description: A unit of the SRM contains 96 different 0.5 mL polypropylene tubes, with approximately 0.010 mg of dehydrated plasmid deoxyribonucleic acid (DNA) in each tube. Depending on the strand transcribed, the controls will mimic either "sense" or "anti-sense" eukaryotic messenger RNA (mRNA). These RNA controls are designed to be used as external, or "spike-in", controls to support confidence in gene expression assays by providing quantitative assessment of the technical performance of a gene expression measurement.
Product Expiration Date: 01 October 2027
Certificate/Report
Revision History:
06 December 2017 ( Change expiration date; Instructions for Use updated; additional FASTA file included; updated references; editorial changes).
NIST Storage Temperature: Freezer (-20 o C)
Please see certificate for user storage information regarding this material.
Safety Information
The certified sequence data file is: SRM2374_Sequence_v1.FASTA.
The file containing the certified confidence estimates for every base of sequence is: SRM2374_Quality_v1.FASTA.
Secondary files are available for accurate and convenient use in sequence mapping applications.
- SRM2374_ambiguities_resolved_v2.FASTA is the sequence datafile with IUPAC ambiguity codes resolved;
- SRM2374_ambiguities_resolved_v1.GTF describes the structures of the RNA controls as if they were genes;
- SRM2374_ambiguities_resolved_NoPolyA_v2.FASTA is the sequence reference file with the polyA tail excluded;
- SRM2374_ambiguities_resolved_NoPolyA_v1.GTFis a GTF with the polyA tail excluded;
- SRM2374_PlasmidVector_by_ERCCNumber_v1.FASTA is the sequence reference file of the plasmid vector.
- SRM2374 _putative_T7_products_NoPolyA_v2.FASTA is the putative sequence generated by in vitro transcription with T7 RNA polymerase and conversion to cDNA, with the polyA tail excluded.
Table(s) of Related Materials DNA Profiling and Nucleic Acid Materials Forensics
Hazard Class: This material is not considered hazardous to ship.
Perishable Types: This material is perishable and ships on cold packs.
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For technical questions regarding this material, please contact McDaniel, Jennifer or SRMs@nist.gov.
For sales inquiry, please contact SRMInfo@nist.gov.